This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.
| Method | HPLC/UHPLC, analytical |
| Brand | NUCLEODUR |
| Base material | NUCLEODUR - high purity silica |
| Phase | NUCLEODUR PFP |
| USP listing | L43 |
| Surface chemistry | Pentafluorophenylpropyl (PFP) modification |
| Mode | Reversed phase (RP) |
| Type | EC UHPLC column (analytical) |
| Hardware | Stainless steel |
| Column length | 50 mm |
| Column inner diameter | 4.6 mm |
| Particle type | Fully porous particles (FPP) |
| Particle shape | Spherical |
| Particle size | 1.8 µm |
| Eluent in HPLC column | Acetonitrile – water |
| Recommended application(s) | Antibiotics, Aromatic and unsaturated compounds, Basic compounds, Especially for polar compounds, Halogenated compounds, Isomers, Pharmaceuticals, Phenols, RP applications UHPLC, Suitable for LC-MS |
| Endcapped | Yes |
| Carbon content | 8.0 % |
| pH stability | 1.0–9.0 |
| Pore size | 110 Å |
| Specific surface according to BET | 340 m²/g |
| Temperature stability (max) | 60 °C |
| Pressure stability (max) | 900 bar / 13050 psi |
| Column volume | 0.83 mL |
| Storage temperature | 15–25 °C / 59–77 °F |
| Scope of delivery | 1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis |
| Gross weight (incl. packaging) | 125.8 g / 0.28 lbs |
| Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
| Hazardous material | No |
