Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.
| Method | HPLC, analytical |
| Brand | NUCLEODUR |
| Base material | NUCLEODUR - high purity silica |
| Phase | NUCLEODUR PolarTec |
| USP listing | L1, L60 |
| Surface chemistry | Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) |
| Mode | Reversed phase (RP) |
| Type | EC HPLC column (analytical) |
| Hardware | Stainless steel |
| Column length | 250 mm |
| Column inner diameter | 4.6 mm |
| Particle type | Fully porous particles (FPP) |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Eluent in HPLC column | Acetonitrile – water |
| Recommended application(s) | Amino acids, Basic pharmaceuticals, Especially for polar compounds, Nitrogen containing compounds, Organic acids, Pesticides, Phenols, Reversed phase, RP applications UHPLC, Suitable for 100 % aqueous eluents, Suitable for LC-MS, Water-soluble vitamins |
| Endcapped | Yes |
| Carbon content | 17.0 % |
| pH stability | 1.0–9.0 |
| Pore size | 110 Å |
| Specific surface according to BET | 340 m²/g |
| Temperature stability (max) | 60 °C |
| Pressure stability (max) | 600 bar / 8700 psi |
| Column volume | 4.15 mL |
| Storage temperature | 15–25 °C / 59–77 °F |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Gross weight (incl. packaging) | 290 g / 0.64 lbs |
| Packaging dimensions | 115 x 400 x 50 mm / 4.53 x 15.75 x 1.97 Inch |
| Hazardous material | No |
