Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.
| Method | HPLC, analytical |
| Brand | NUCLEODUR |
| Base material | NUCLEODUR - high purity silica |
| Phase | NUCLEODUR HILIC |
| USP listing | Not available or proprietary |
| Surface chemistry | Ammonium – sulfonic acid (zwitterionic) modification |
| Mode | Hydrophilic Interaction Chromatography (HILIC) |
| Type | EC HPLC column (analytical) |
| Hardware | Stainless steel |
| Column length | 250 mm |
| Column inner diameter | 2 mm |
| Particle type | Fully porous particles (FPP) |
| Particle shape | Spherical |
| Particle size | 3 µm |
| Eluent in HPLC column | Acetonitrile – water |
| Recommended application(s) | Amino acids, Highly polar analytes, HILIC applications HPLC, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins |
| Endcapped | No |
| Carbon content | 7.0 % |
| pH stability | 2.0–8.5 |
| Pore size | 110 Å |
| Specific surface according to BET | 340 m²/g |
| Temperature stability (max) | 60 °C |
| Pressure stability (max) | 600 bar / 8700 psi |
| Column volume | 0.8 mL |
| Storage temperature | 15–25 °C / 59–77 °F |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Gross weight (incl. packaging) | 255.47 g / 0.56 lbs |
| Packaging dimensions | 405 x 117 x 54 mm / 15.94 x 4.61 x 2.13 Inch |
| Hazardous material | No |
