Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.
Method | HPLC/UHPLC, analytical |
Brand | NUCLEODUR |
Base material | NUCLEODUR - high purity silica |
Phase | NUCLEODUR PolarTec |
USP listing | L1, L60 |
Surface chemistry | Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) |
Mode | Reversed phase (RP) |
Type | EC UHPLC column (analytical) |
Hardware | Stainless steel |
Column length | 100 mm |
Column inner diameter | 2 mm |
Particle type | Fully porous particles (FPP) |
Particle shape | Spherical |
Particle size | 1.8 µm |
Eluent in HPLC column | Acetonitrile – water |
Recommended application(s) | Amino acids, Basic pharmaceuticals, Especially for polar compounds, Nitrogen containing compounds, Organic acids, Pesticides, Phenols, RP applications UHPLC, Suitable for 100 % aqueous eluents, Suitable for LC-MS, Water-soluble vitamins |
Endcapped | Yes |
Carbon content | 17.0 % |
pH stability | 1.0–9.0 |
Pore size | 110 Å |
Specific surface according to BET | 340 m²/g |
Temperature stability (max) | 60 °C |
Pressure stability (max) | 900 bar / 13050 psi |
Column volume | 0.3 mL |
Storage temperature | 15–25 °C / 59–77 °F |
Scope of delivery | 1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis |
Gross weight (incl. packaging) | 149.33 g / 0.33 lbs |
Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
Hazardous material | No |